Dr Pwee Keng Ho obtained his MBBS in 1991 and his MMed (Public Health) in 1999 from the National University of Singapore. He has been working in public health and health administration since 1995, first in the Ministry of Health, Singapore, and since 2015, in Changi General Hospital.
Since 2002, Dr Pwee has been working in the field of health technology assessment. He also worked in clinical practice guidelines development during his time at the Ministry of Health. Dr Pwee was a member of the Board of Directors of Health Technology Assessment International (HTAi) from 2010 to 2014, and was a co-opted member of the Board of Trustees of the Guidelines International Network from 2010 to 2011. In 2024, Dr Pwee was appointed Chair of the Practice Guidelines Committee of the Academy of Medicine, Singapore.